Regulatory

Part:BBa_K3612014:Design

Designed by: Jesus Durand, Matias Rojas, Diego Benites, Maria Castromonte.   Group: iGEM20_UPCH_Peru   (2020-10-20)


Pseudoalteromonas constitutive promoter (Pasp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence optimized for Pseudoalteromonas nigrifaciens.


Source

Part of cold-adapted aspC(coding for aspartate aminotransferase from P. haloplanktis TAC125): AJ001736.

References

(1) Sannino F, Giuliani M, Salvatore U, Apuzzo GA, de Pascale D, Fani R, et al. A novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125. Appl Microbiol Biotechnol. 2017 Jan 27;101(2):725–34.

(2) Parrilli E, Tutino ML. Psychrophiles: From Biodiversity to Biotechnology: Second Edition. Psychrophiles From Biodivers to Biotechnol Second Ed. 2017;1–685.

(3) Papa R, Rippa V, Sannia G, Marino G, Duilio A. An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125. J Biotechnol. 2007 Jan 1;127(2):199–210.

(4) Yu ZC, Tang BL, Zhao DL, Pang X, Qin QL, Zhou BC, et al. Development of a cold-adapted Pseudoalteromonas expression system for the Pseudoalteromonas proteins intractable for the Escherichia coli system. PLoS One. 2015;10(9):1–14.

(5) Tutino, M. L., Parrilli, E., Giaquinto, L., Duilio, A., Sannia, G., Feller, G., & Marino, G. (2002). Secretion of alpha-Amylase from Pseudoalteromonas haloplanktis TAB23: Two Different Pathways in Different Hosts. Society, 184(20), 5814–5817.

(6) Birolo L, Tutino LM, Fontanella B, Gerday C, Mainolfi K, Pascarella S, et al. Aspartate aminotransferase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125. Cloning, expression, properties, and molecular modelling. Eur J Biochem. 2000 May;267(9):2790–802.